Professor Michael Bushell
About
Biography
I am an Emeritus Professor of Microbiology in the Microbial Sciences Department, where I was formerly Head of Department.
Prior to joining the University, I worked in the Pharmaceutical industry (Pfizer and GSK).
I did my PhD with Alan Bull at the University of Kent on the physiology of Aspergillus nidulans in chemostat culture and my first degree (in Microbiology) at King's College London.
I currently carry out ad hoc projects for the University and BBSRC but my main professional activity is membership of the Advisory Committee on Novel Foods and Processes (ACNFP) of the Food Standards Agency. I have been a member for 10 years, having been seconded for the past three years to help to establish new post-Brexit procedures. My main contribution to the panel is to advise on bioprocess analysis, and genomic/bioinformatics driven evaluation of applications for marketing of novel food products in the UK.
Areas of specialism
Business, industry and community links
Publications
Selected publications:Recent:Beste DJV, Laing E, Bonde B, Avignone-Rossa C, Bushell ME, McFadden JJ (2007) Transcriptomic analysis identifies growth rate modulation as a component of the adaptation of mycobacteria to survival inside the macrophage. Journal of Bacteriology 189 3969-3976
Bushell ME, Sequeira SIP, Khannapho C, Zhao HJ, Chater, Butler MJ, Kierzek AM, Avignone-Rossa CA (2006) Enzyme and Microbial Technology.39 (6): 1347-1353
Cattini N, Laing E, Allenby N, Bucca G, Bushell ME, Kierzek A, Smith C (2007) Analysis of gene expression in the context of the genome scale metabolic network provides insight into the metabolic and phenotypic switch to antibiotic production production by Streptomyces coelicolor 14th International conference on the biology of Actinomycetes Abstract book p.93
Ahmed AEI, Hay JN, Bushell ME, Wardell JN, Cavalli G, (2007) Biocidal Polymers (I): Preparation and biological activity of some novel biocidal polymers based on Uramil and its Azo-dyes, Reactive & Functional Polymers doi: 10.1016/j.reactfunctpolym.2007.09.004
Beste DJV, Hooper T, Stewart G, Bonde B, Avignone-Rossa C, Bushell ME, Wheeler P, Klamt S, Kierzek AM , McFadden J (2007) GSMN-TB: a web-based genome scale network model of Mycobacterium tuberculosis metabolism. Genome Biology 8(5): Art. No. r89
Of importance to current activities:Ives, P.R. and Bushell, M.E. (1997). Manipulation of the physiology of clavulanic acid production in Streptomyces clavuligerus. Microbiology, 143, 3573-3579.
Wardell, J.N. and Bushell, M.E. (1999) The kinetics and manipulation of hyphal breakage and its effect on antibiotic production. Enzyme and Microbial Technology 25, 404-410.
Bushell, M.E. & Bull, A.T. (1999) Sporulation at minimum specific growth rate in Aspergillus nidulans chemostat culture predicted using protein synthesis efficiency estimations. J. Basic Microbiol. 39, 293-298.
Dunstan, GH, AvignoneRossa, C, Langley, D and Bushell, ME (2000) The Vancomycin biosynthetic pathway is induced in oxygen-limited Amycolatopsis orientalis (ATCC 19795) cultures that do not produce antibiotic. Enzyme and microbial technology, 2000, 27, 502-510
Samantha Kirk, Claudio A. Avignone-Rossa, Michael E. Bushell. (2000) Growth limiting substrate affects antibiotic production and associated metabolic fluxes in Streptomyces clavuligerus. Biotechnology Letters 22 pp. 1803-1809
J N Wardell, S M Stocks, C R Thomas M E Bushell (2002) Decreasing the hyphal branching rate of Saccharopolyspora erythreae NRRL 2338 leads to increased resistance to breakage and increased antibiotic production. Biotechnology and Bioengineering 78. 141-146.M E Bushell, M Rowe, C A Avignone-Rossa J N Wardell (2003) Cyclic fed batch culture for production of human serum albumin in Pichia pastoris. Biotechnology and Bioengineering 82. 678-683.
Rozkov A, Avignone-Rossa CA, Ertl PF, Jones P, O'Kennedy RD, Smith JJ, Dale JW, Bushell ME (2004) Characterization of the metabolic burden on Escherichia coli DH1 cells imposed by the presence of a plasmid containing a gene therapy sequence. Biotechnology and Bioengineering 88: 909-915
D. J. V. Beste,1 J. Peters,1 T. Hooper,1 C. Avignone-Rossa,1 M. E. Bushell,1 and J. McFadden1* (2005). Compiling a Molecular Inventory for Mycobacterium bovis BCG at Two Growth Rates: Evidence for Growth Rate-Mediated Regulation of Ribosome Biosynthesis and Lipid Metabolism. Journal of Bacteriology. 1677-1684, Vol. 187, No. 5
Bushell ME, Kirk S, Zhao H and Avignone-Rossa CA (2006) Manipulation of the physiology of clavulanic acid biosynthesis with the aid of metabolic flux analysis. Enz Microb Technol 39, 149-157.
Rozkov, A, Avignone-Rossa C.A., Ertl P.F., Jones P., O'Kennedy R.D., Smith J.J., Dale J.W. Bushell M.E.(2006) Fed batch culture with declining specific growth rate for high-yielding production of a plasmid containing a gene therapy sequence in Escherichia coli DH1. Enz Microb Technol 39, 47-50.
Bushell, ME, Sequeira, SIP, Khannapho, C, Zhao, H, Chater, KF, Butler, MJ, Kierzek, AM, Avignone-Rossa, CA (2006) The use of genome scale metabolic flux variability analysis for process feed formulation based on an investigation of the effects of the zwf mutation on antibiotic production in Streptomyces coelicolor. Enz Microb Technol doi:10.1016/j.enzmictec.2006.06.011