Professor Costas Ioannides

Emeritus Professor of Mechanistic Toxicology
+44 (0)1483 689709
34 AY 03

About

Chemopreventive phytochemicals, in particular glucosinolates/isothiocyanates. Interactions between chemical carcinogens. Precision-cut tissue slices.

Biography

Employment Record:

Jan.1973-Aug.1973: Postdoctoral Research Fellow. Department of Biochemistry, University of Surrey:

Developmental toxicology in ferrets, funded by the World Health Organisation.

1973-1974: Postdoctoral Research Fellow, Department of Biological Chemistry, University of Athens:

Pharmacokinetics and metabolism of feprazone in the rat and man, funded by

Istituto de Angeli, Milan, Italy.

1974-1976: Postdoctoral Research Fellow, Department of Biochemistry, University of Surrey:

Metabolism and toxicity of organochlorine insecticides in the neonatal and adult ferret,

supported by the World Health

Organisation; toxicity of feprazone financed by Istituto de Angeli, Milan; and ulcer-healing

properties of gefarnate, funded by Boehringer Ingelheim, West Germany.

1975-1976: Part-time Lecturer, Centre of Adult Education, University of Surrey:

Organiser and Lecturer of a course of 40 hours on the "Use and Abuse of Drugs".

Sep.1976-Mar.1977: Temporary Lecturer, Department of Biochemistry, University of Surrey:

Lecturing in Basic Biochemistry, Pharmacological Biochemistry, Medical Biochemistry

and Forensic Science.

1977-1978: Head of Drug Metabolism, Battelle Research Institute, Geneva, Switzerland.

1978-1981: Experimental Officer, Department of Biochemistry, University of Surrey,

responsible directly to the Head of Department.

1981-1992: Senior Experimental Officer, Department of Biochemistry, University of Surrey.

1978-2008: Honorary Visiting Lecturer in Biochemical Pharmacology, Royal Holloway College,

University of London: Lectures (13) to final year undergraduate students on

pharmacokinetics and the principles of toxicity.

1992-2003: Senior Research Fellow, School of Biological Sciences, University of Surrey.

1993-2008 Associate Editor, Xenobiotica

2003- to date: Professor in Mechanistic Toxicology, Faculty of Health and Medical Sciences,

University of Surrey.

2008- to date Editor-in-Chief, Xenobiotica

Research interests

Research collaborations

(1) Research Center for Industrial Corps, Agricultural Research Council, (CRA-CIN), Bologna, Italy (2) Investigador Cientifico del CSIC, Instituto de Investigaciones Quimicas, Seville, Spain (3) ATEI Thessaloniki Faculty of Agricultural Technology, Department of Animal Production Laboratory of Reproduction, Thessaloniki, Greece (4) Heriot-Watt University, Scotland

Affiliations

International Society for the Study of Xenobiotics

British Society of Toxicology

Additional Information

Editor-in-Chief of 'Xenobiotica'.

Publications

  • Lake BG, Vickers AEM and Ioannides C (2013) Preface. Xenobiotica, 43:1

    • 2. Ioannides C (2013) Up-regulation of cytochrome P450 and phase II enzymes by xenobiotics in precision-cut tissue slices. Xenobiotica, 43, 15-28

  • Abdull Razis AF, De Nicola GR, Pagnotta E, Iori C and Ioannides C (2013) A glucosinolate-rich extract of Japanese Daikon perturbs carcinogen-metabolizing enzyme systems in rat, being a potent inducer of hepatic glutathione S-transferase. Eur J Nutr, 52, 1279-1285

  • Ioannides C and Lake BG (2012) Precision-cut tissue slices: A suitable in vitro system for the study of the induction of drug-metabolizing enzyme systems. In Encyclopedia of Drug Metabolism and Interactions, Volume 6, Lyubimov AV (Ed), John Wiley & Sons, Inc., pp. 1-26.

  • Abdull Razis AF, Hanlon N, Soltys E, Krizova V, Iori R, Plant KE, Plant N and Ioannides C (2012) The naturally occurring aliphatic isothiocyanates sulforaphane and erucin are weak agonists but potent non-competitive antagonists of the aryl hydrocarbon receptor. Arch. Toxicol., 86, 1505-1514.

  • Abdull Razis AF, Bagatta M, De Nicola GR, Iori R, Plant N and Ioannides C (2012) Characterization of the temporal induction of hepatic xenobiotic-metabolizing enzymes by glucosinolates and isothiocyanates: Requirement for at least a 6 h exposure to elicit complete induction profile. J Agric Food Chem, 60, 5556-5564.

  • Abdull Razis AF, Konsue N, Dervetzoglou M, Plant KE, Plant N and Ioannides C (2012) Phenethyl isothiocyanate, a naturally-occurring phytochemical, is an antagonist of the aryl hydrocarbon receptor. Mol. Nutr. Food Res., 56, 425-434.

  • Cooke BR, Bligh SWA, Cybulski ZR, Ioannides C and Hall M (2012) Debrisoquine metabolism and CYP2D expression in marmoset liver microsomes. Drug Met. Disp., 40, 70-75.

  • Abdull Razis AF, De Nicola G, Pagnotta E, Iori R and Ioannides C (2012) 4-Methysulfanyl-3-butenyl isothiocyanate derived from glucoraphasatin is a potent inducer of rat hepatic phase II enzymes and a potential chemopreventive agent. Arch. Toxicol., 86, 183-194.

  • Abdull Razis AF, Iori R and Ioannides C (2011) The natural chemopreventive phytochemical R-sulforaphane is a far more potent inducer of the carcinogen-detoxifying enzyme systems in rat liver and lung than the S-isomer. Intern. J. Cancer, 128, 2775-2782.

  • Abdull Razis AF, Bagatta M, De Nicola GR, Iori R and Ioannides C (2011) Induction of epoxide hydrolase and glucuronosyl transferase by isothiocyanates and intact glucosinolates in precision-cut rat liver slices: importance of side-chain substituent and chirality. Arch. Toxicol., 85, 919-927.

  • Hanlon N, Konsue N, Coldham N, Sauer MJ and Ioannides C (2011) Exposure to isothiocyanates suprresses urinary mutagenicity in rats treated with the heterocyclic amine IQ: Lack of association with CYP1 activity. Nut. Cancer, 63, 300-305.

  • Abdull Razis AF, Bagatta M, De Nicola GR, Iori R and Ioannides C (2011) Up-regulation of cytochrome P450 and Phase II enzyme systems in rat precision-cut rat lung slices by the intact glucosinolates, glucoraphanin and glucoerucin. Lung Cancer, 71, 298-305.

  • Abdull Razis AF, Bagatta M, De Nicola GR, Iori R and Ioannides C (2010) Intact glucosinolates modulate hepatic cytochrome P450 and phase II conjugation activities and may contribute directly to the chemopreventive activity of cruciferous vegetables. Toxicology, 277, 74-85.

  • Konsue N and Ioannides C (2010) Differential response of four human livers to modulation of phase II enzyme systems by the chemopreventive phytochemical phenethyl isothiocyanate. Molec. Nut. Food Res., 54, 1477-1485.

  • Ioannides C, Hanlon N, Konsue N (2010) Isothiocyanates: A chemical class of potential nutraceuticals. J Neutraceut., 3, 55-62.

  • Konsue N and Ioannides C (2010) Phenethyl isocyanate is not the metabolite of phenethyl isothiocyanate responsible for mechanism-based inhibition of cytochrome P450. Arch. Toxicol., 84, 751-759.

  • Konsue N and Ioannides C (2010) Modulation of carcinogen-metabolising cytochromes P450 in human liver by the chemopreventive phytochemical phenethyl isothiocyanate, a constituent of cruciferous vegetables. Toxicology, 268, 184-190.

  • Konsue N, Kirkpatrick J, Kuhnert N, King LJ and Ioannides C (2010) Repeated oral administration modulates the pharmacokinetic behaviour of the chemopreventive agent phenethyl isothiocyanate in rats. Molecular Nutrition & Food Research, 54, 426-432.

  • Hanlon N, Coldham N, Gielbert A, Sauer MJ and Ioannides C (2009) Repeated intake of broccoli does not lead to higher plasma levels of sulforaphane in human volunteers. Cancer Lett., 284, 15-20.

  • Ioannides C (2009) Xenobiotic metabolism. In:Wiley Encyclopedia of Chemical Biology, John Wiley & Sons, Hoboken, pp 721-731.

  • Lopez-Mendez C, Bermudez-Fajardo A, Ioannides C and Ernesto Oviedo-Orta E (2009) Effect of 2-amino-9H-pyrido [2,3-b] indole (AαC), a carcinogenic heterocyclic amine present in food, on atherosclerotic plaque development in apoE deficient mice. Toxicol. Letters, 185, 73-78.

  • Hanlon N, Poynton CL, Coldham N, Sauer MJ and Ioannides C (2009) The aliphatic isothiocyanates erucin and sulforaphane do not effectively up-regulate NAD(P)H: quinone oxidoreductase (NQO1) in human liver compared with rat. Molecular Nutrition & Food Research, 53, 836-844.

  • Hanlon N, Coldham N, Sauer MJ and Ioannides C (2009) Modulation of rat pulmonary carcinogen-metabolising enzyme systems by the isothiocyanates erucin and sulforaphane. Chem-Biol. Interactions, 177, 115-120.

  • Konsue N and Ioannides C (2008) Tissue differences in the modulation of rat cytochromes P450 and phase II conjugation systems by dietary doses of phenethyl isothiocyanate, Fd. Chem. Toxicol.46, 3677-3683

  • Hanlon N, Coldham N, Sauer MJ and Ioannides C (2008) Up-regulation of the CYP1 family in rat and human liver by the aliphatic isothiocyanates erucin and sulforaphane. Toxicology, 252, 92-98.

  • Staal YCM, Pushparajah DS, van Herwingen MHM, Gottschalk RWH, Maas LM, Ioannides C, van Schooten FJ, van Delft J (2008) "Interactions between polycyclic aromatic hydrocarbons in binary mixtures: Effects on gene expression and DNA adduct formation in precision-cut rat liver slices. Mutagenesis. 23, 491-499.

  • Hanlon N, Okpara M, Coldham N, Sauer MJ and Ioannides C (2008) Modulation of rat hepatic and pulmonary cytochromes P450 and Phase II enzyme systems by erucin, an isothiocyanate structurally related to sulforaphane. Journal of Agriculture and Food Chemistry. 56, 7866-7871

  • Pushparajah DS, Umachandran M, Plant KE, Plant N and Ioannides C (2008) Up-regulation of the glutathione S-transferase system in human liver by polycyclic aromatic hydrocarbons; comparison with rat liver and lung. Mutagenesis. 23, 68-80.

  • Pushparajah DS, Umachandran M, Plant KE, Plant N and Ioannides C (2008) Differential response of human and rat epoxide hydrolase to polycyclic aromatic hydrocarbon exposure; studies using precision-cut tissue slices. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 640, 153-161.

  • Hanlon N, Coldham N, Gielbert A, Kuhnert N, Sauer MJ, King LJ and Ioannides C (2008) Absolute bioavailability and dose-dependent pharmacokinetic behaviour of dietary doses of the chemopreventive isothiocyanate sulforaphane in the rat. British Journal of Nutrition. 99, 559-564

  • Ioannides C (2008) Cytochromes P450 in the metabolism and bioactivation of chemicals. In: Chemistry and Molecular Aspects of Drug Design and Action, Rekka EA and Kourounakis PN (Eds), CRC Press, Boca Raton, pp. 253-271.

  • Pushparajah DS, Umachandran M, Nazir T, Plant KE, Plant N, Lewis DFV and Ioannides C (2008) Up-regulation of CYP1A/B in rat lung and liver, and human precision-cut slices by a series of polycyclic aromatic hydrocarbons; association with the Ah locus and importance of molecular size. Toxicology In Vitro, 22, 128-145.

  • Ioannides C (2007) Bioactivation of chemicals by cytochromes P450. Environm. Biotechnol. 3, 1-9

  • Pushparajah, DS, Umachandran, M, Plant, KE, Plant, N and Ioannides, C (2007) Evaluation of the precision-cut liver and lung slice systems for the study of induction of CYP1, epoxide hydrolase and glutathione S-transferase activities. Toxicology, 231, 68-80.

  • Staal, YCM, van Herwijnen, MHM, Pushparajah, DS, Umachandran, M, Ioannides, C, van Schooten FJ and van Delft JHM (2007) Modulation of gene expression and DNA adduct formation in precision-cut liver slices exposed to polycyclic aromatic hydrocarbons of different carcinogenic potency. Mutagenesis, 22, 55-62.

  • Umachandran, M and Ioannides C (2006) Stability of cytochromes P450 and phase II conjugation systems in precision-cut lung slices cultured up to 72h. Toxicology, 224, 14-21.

  • Ioannides C (2006) Cytochrome P450 expression in the liver of food-producing animals. Current Drug Met., 7, 335-348.

  • Skene, DJ, Timbers, SE, Middleton, B, English J, Kopp C, Tobler, I and Ioannides, C (2006) Mice convert melatonin to 6-sulphatoxymelatonin. General and Comparative Endocrinology, 147, 371-376.

  • Ioannides C (2005) Anticarcinogenic potential of black tea: Studies in animals and humans. In:Nutraceuticals and Food Safety for well-being life, Proceedings of the International Symposium and Annual Meeting of the Korean Society of Food Science and Nutrition, pp. 23-29.

  • Yoxall, V, Kentish, P, Coldham N, Kuhnert N, Sauer MJ and Ioannides C (2005) Modulation of cytochromes P450 and phase II enzymes by dietary doses of sulforaphane in rats: Implications for its chemopreventive activity. Intern. J. Cancer, 117, 356-362.

  • Lewis, DFV and Ioannides, C (2005) The importance of cytochromes P450 in chemical toxicity: structure-activity considerations. Current Topics in Toxicology, 2, 25-32.

  • Yoxall, V, Umachandran, V, Wilson J, Kentish, P and Ioannides C (2005) Black tea modulates the excretion of urinary mutagens in rats exposed to 6-aminochrysene: induction of cytochromes P450 by 6-aminochrysene in the rat. Mutagenesis, 20, 23-28.

  • Yoxall, VR, Bishop J and Ioannides C (2004) Effect of black tea intake on the excretion of mutagens in the urine of volunteers taking a beef meal. Cancer Epidemiology, Biomarkers and Prevention, 13, 2196-2202.

  • Umachandran M, Howarth J and Ioannides C (2004) Metabolic and structural viability of precision-cut rat lung slices in culture. Xenobiotica, 34, 771-780.

  • Ioannides C and Lewis DFV (2004) Cytochromes P450 in the bioactivation of chemicals. Current Topics in Medicinal Chemistry, 4, 1767-1788.

  • Yoxall VR, Parker DA, Kentish PA and Ioannides C (2004) Short-term black tea intake modulates the excretion of urinary mutagens in IQ-treated rats: Role of CYP1A2 up-regulation. Arch. Toxicol., 78. 477-482.

  • Sivapathasundaram S, Howells LC, Sauer MJ and Ioannides C (2004) Functional integrity of precision-cut liver slices from deer and cattle, J. Vet. Pharmacol. Ther., 27, 79-84.

  • Yoxall V, Wilson J and Ioannides C (2004) An improved method for the extraction of mutagens from human urine and cooked meat using blue rayon, Mut. Res., 559, 121-130.

  • Catterall F, Kassimi AI, Clifford MN and Ioannides C (2003) Influence of milk on the antimutagenic potential of green and black teas. Anticancer Res., 23, 3863-3868.

  • Ioannides C and Yoxall V (2003) Antimutagenic activity of tea: role of polyphenols, Curr. Opin. Clin. Nutr. Metab. Care, 6, 649-656.

  • Catterall F, King LJ, Clifford MN and Ioannides C (2003) Bioavailability of dietary doses of 3H-labelled antioxidants (+)-catechin and (-)-epicatechin in the rat, Xenobiotica, 33, 743-753.

  • Catterall F., McArdle NJ, Mitchell L, Papayanni A, Clifford MN and Ioannides C (2003) Hepatic and intestinal cytochrome P450 and conjugase activities in rats treated with black tea theafulvins and theaflavins. Fd. Chem. Toxicol., 41, 1141-1147.

  • Sivapathasundaram S, Magnisali P, Coldham NG, Howells LC, Sauer MJ and Ioannides C (2003) Cytochrome P450 expression and testosterone metabolism in the liver of deer. Toxicology, 187, 49-65.

  • Lewis DFV, Ioannides C and Parke DV (2003) A quantitative structure-activity relationship (QSAR) study of mutagenicity in several series of organic chemicals to be activated by cytochrome P450 enzymes. Teratogenesis, Carcinogenesis and Mutagenesis, Supplement 1, 187-193.

  • Catterall F, Copeland E, Clifford MN and Ioannides C (2003) Effects of black tea theafulvins on aflatoxin B1mutagenesis in the Ames test. Mutagenesis, 18, 145-150.

  • Sivapathasundaram S, Sauer MJ and Ioannides C (2003) Xenobiotic conjugation systems in deer compared with cattle and rat. Compar. Biochem. Physiol., 124, 169-173.

  • Ioannides C (2003) Drug-phytochemical interactions. Inflammopharmacology, 11, 7-42.

  • Cattterall F, King, LJ and Ioannides C (2002) Mutagenic activity of the glutathione S-transferase substrate 1-chloro-2,4-dinitrobenzene (CDNB) in the Salmonella mutagenicity assay. Mutation Res., 520, 119-124.

  • Ioannides C (2002) Xenobiotic metabolism: an overview. In: Enzyme Systems that Metabolise Drugs and Other Xenobiotics, Ioannides C (Ed), John Wiley & Sons, Chichester, pp. 1-32.

  • Dhawan A, Anderson D, de Pascual-Teresa S, Santos-Buelga C, Clifford MN and Ioannides C (2002) Evaluation of the antigenotoxic potential of monomeric and dimeric flavanols, and black tea polyphenols against heterocyclic amine-induced DNA damage in human lymphocytes using the Comet assay. Mutation Res., 515, 39-56.

  • Ioannides C (2002) Pharmacokinetic interactions between herbal remedies and medicinal drugs. Xenobiotica, 32, 451-478.

  • Skene DJ, Papapagiannidou E, Hashemi E, Snelling J, Lewis DFV, Fernandez M and Ioannides C (2001) Contribution of CYP1A2 in the hepatic metabolism of melatonin: studies with isolated microsomal preparations and liver slices. J. Pineal Res., 31, 333-342.

  • Sivapathasundaram S, Magnisali P, Coldham NG, Howells LC, Sauer MJ and Ioannides C (2001) A study of the expression of the xenobiotic-metabolising cytochrome P450 proteins and of testosterone metabolism in bovine liver. Biochem. Pharmacol., 62, 635-645.

  • Catterall F, Coombs MM, Ioannides C, Sepiol JJ and Wilson J (2001) Mutagenicity of bay-region amino-substituted cyclopenta[a]phenanthrenes and 2- and 5-aminochrysene. Mutation Research, 492, 7-11.

  • Walton K, Coombs MM, Walker R and Ioannides C (2001) The metabolism and bioactivation of agaritine and other mushroom hydrazines by whole mushroom homogenate and by mushroom tyrosinase. Toxicology, 161, 165-177.

  • Anderson D, Dhawan A, Yardley-Jones A, Ioannides C and J. Webb J (2001) Effect of antioxidant flavonoids and a food mutagen on lymphocytes of a thalassaemia patient without chelation therapy in the Comet assay. Teratogenesis, Carcinogenesis and Mutagenesis, 21, 165-174.

  • Lewis DFV, Ioannides C, Schulte-Hermann R and Parke DV (2000) Quantitative structure-activity relationships in a series of endogenous and synthetic steroids exhibiting induction of CYP3A activity and hepatomegaly associated with increased DNA synthesis. J. Steroid Biochem. Molec. Biol., 74, 179-185.

  • Hashemi E, Till C and Ioannides C (2000) Stability of cytochrome P450 in cultured precision-cut rat liver slices. Toxicology, 149, 51-61.

  • Catterall F, Coombs MM, Ioannides C and Walton K (2000) Bioactivation of the carcinogen 11-methoxy-16,17-dihydro-15H-cyclopenta[a]phenanthrene. Mutat. Res., 465, 85-90.

  • Walton K, Coombs MM, King LJ, Walker R and Ioannides C (2000) The fate of the mushroom hydrazine, agaritine, in the rat and mouse. Nutrition and Cancer, 37, 55-64.

  • Catterall F, Souqet J-M, Cheynier V, de Pascual-Teresa S, Santos-Buelga C, Clifford MN and Ioannides C (2000) Differential modulation of the genotoxicity of food carcinogens by naturally occurring monomeric and dimeric polyphenolics. Environ. Molec. Mutagen., 35, 86-98.

  • Ioannides C (2000) Xenobiotic metabolism and bioactivation by cytochromes P450. In: Biomolecular Injury: Causes and prevention of toxicity. Wiseman H, Goldfarb P, Ridgway T and Wiseman A (eds), John Wiley & Sons, 103-144.

  • Catterall F, Souquet J-M, Cheynier V, Clifford MN and Ioannides C (2000) Modulation of the mutagenicity of food carcinogens by oligomeric and polymeric procyanidins isolated form grape seeds: synergistic genotoxicity with N-nitrosopyrrolidine. J. Sci. Fd. Agri., 80, 91-101.

  • Barnett CR and Ioannides C (2000) Xenobiotic metabolizing enzyme systems and aging. Methods Mo.l Med., 38, 119-130.