Anna Salvian
Academic and research departments
Department of Microbial Sciences, School of Chemistry and Chemical Engineering.About
My research project
Development of microbial self-powered biosensor for water quality monitoringWater pollution has increasingly been recognised as a global issue, as it poses a serious threat to both human and environmental health. Microbial fuel cells (MFCs) are a novel technology in which microorganisms oxidise biodegradable organic materials in wastewater to generate electricity. They may be used as biosensors to evaluate the biochemical oxygen demand (BOD) in the feed water in real-time, with the added benefit of being self-sustaining. The goal of my research is to develop a cost-effective and operationally stable MFC-based biosensor for applications in water quality monitoring.
Supervisors
Water pollution has increasingly been recognised as a global issue, as it poses a serious threat to both human and environmental health. Microbial fuel cells (MFCs) are a novel technology in which microorganisms oxidise biodegradable organic materials in wastewater to generate electricity. They may be used as biosensors to evaluate the biochemical oxygen demand (BOD) in the feed water in real-time, with the added benefit of being self-sustaining. The goal of my research is to develop a cost-effective and operationally stable MFC-based biosensor for applications in water quality monitoring.
University roles and responsibilities
- Chemical and Process Engineering PGR Course Rep
Publications
The increasing global water pollution leads to the need for urgent development of rapid and accurate water quality monitoring methods. Microbial fuel cells (MFCs) have emerged as real-time biosensors for biochemical oxygen demand (BOD), but they grapple with several challenges, including issues related to reproducibility, operational stability, and cost-effectiveness. These challenges are substantially shaped by the selection of an appropriate air-breathing cathode. Previous studies indicated a critical influence of the cathode on both the enduring electrochemical performance of MFCs and the taxonomic diversity at the electroactive anode. However, the effect of different gas diffusion electrodes (GDE) on 3D-printed single-chamber MFCs for BOD biosensing application and its effect on the bioelectroactive anode was not investigated before. Our study focuses on comparing GDE cathode materials to enhance MFC performance for precise and rapid BOD analysis in wastewater. We examined for over 120 days two Pt-coated air-breathing cathodes with distinct carbonaceous gas diffusion layers (GDLs) and catalyst layers (CLs): cost-effective carbon paper (CP) with hand-coated CL and more expensive woven carbon cloth (CC) with CL pre-applied by the supplier. The results show significant differences in electrochemical characteristics and anodic biofilm composition between MFCs with CP and CC GDE cathodes. CP-MFCs exhibited lower sensitivity (16.6 C L mg-1 m-2) and a narrower dynamic range (25 to 600 mg L-1), attributed to biofouling-related degradation of the GDE. In contrast, CC-MFCs demonstrated superior performance with higher sensitivity (37.6 C L mg-1 m-2) and a broader dynamic range (25 to 800 mg L-1). In conclusion, our study underscores the pivotal role of cathode selection in 3D-printed MFC biosensors, influencing anodic biofilm enrichment time and overall BOD assessment performance. We recommend the use of cost-effective CP GDL with hand-coated CL for short-term MFC biosensor applications, while advocating for CC GDL supplied with CL as the preferred choice for long-term sensing implementations with enduring reliability.
Efficient wastewater treatment monitoring is vital for addressing water scarcity. Microbial fuel cells (MFCs) have emerged as real-time biosensors for biochemical oxygen demand (BOD) in urban wastewater. Discrepancies in signal generation may arise due to changes in the composition and metabolism of mixed-culture electroactive biofilms stemming from different wastewater compositions. In this study, 3D-printed MFC-based biosensors were employed to assess the BOD of sterile complex artificial wastewater and untreated urban wastewater. Alterations in the microbial composition of the anode were evaluated using 16S rRNA sequencing and metagenomics analysis. Results show that MFC-based biosensors can be effectively recalibrated for diverse types of wastewater, maintaining consistent sensitivity (0.64 ± 0.10 mA L mg −1 m −2 with synthetic wastewater and 0.78 ± 0.13 mA L mg −1 m −2 with urban wastewater) and limit of detection (49 ± 8 mg L −1 for synthetic wastewater and 44 ± 7 mg L −1 for urban wastewater). Crucially, pre-sterilization, conductivity adjustments, and nitrogen purging of wastewater are not required before its introduction into the biosensor. However, the presence of native aerobic microorganisms in the wastewater might affect the current output. Metagenomics and taxonomic analyses revealed that the alterations in biofilm composition are predominantly in response to the varied chemical and microbiological compositions of different substrates. Despite variations in anodic biofilm composition, the MFC-based biosensor maintains a relative error comparable to the standard BOD test. This highlights the resilience and flexibility of the biosensor when directly used with a variety of wastewater types before full biofilm adjustment.