Understanding the Host-Pathogen interaction of PRRSV

Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of the accordingly named syndrome in pigs, which remains one of the most important viruses and diseases in pigs. While in vitro models of PRRSV-1 infection have made progress experimental in vivo studies are required to further understand the complex nature of syndromic diseases through systems immunology approaches.

An intriguing feature of PRRS is the late appearance of neutralising antibodies – occasionally long after the infection has been cleared. This emphasises the dominant role of the innate immune system and T cells in the host immune response against PRRSV, which are not fully understood. 

The main objective of the project is to study the host-pathogen interaction of PRRSV-1 with the host immune system in pigs. While PRRSV-1 can be resolved by the host before the appearance of neutralising antibodies in blood, a role for the adaptive immune system is envisaged. The approaches to study the host-pathogen interaction of PRRSV-1 shall include the: 

• Analysis of the T and B cell repertoire and function induced upon vaccination or PRRSV-1 infection. To this end, the study will: 
  • determine the appearance of nAbs
  • utilise B and T cell receptor (BCR/TCR) sequencing in the local secondary lymphoid over time 
  • use In vitro systems to demonstrate the functionality of antibodies 
• It is envisaged that in silico systems can be used for further analysis of the heterogeneity of receptors identified 

This project will use multicolour flow cytometry in combination with cell culture, RNA sequencing (RNA-Seq) and scRNA Seq techniques. 

You will need to meet the minimum entry requirements for our Veterinary Medicine and Science PhD programme. 

Open to UK nationals and those who pay UK/home rate fees. See UKCISA for further information

In addition, you will have to be resident in the UK for at least two, ideally 3 years (at the time of application) to meet the requirement for additional security clearances required to carry out work as part of this project.